Library sample preparation

• Prepare a total amount of 400 fmol in 20 μL of nuclease-free water or low TE buffer (this translates to approximately 260 ng for a 1 kb amplicon).
• Submit samples in 96-well PCR plates or in tube strips with the number indicating the sample number of each strip.
• For these samples we do recommend Fluorometric Quantification (e.g. Qubit) if possible.
• Please make sure your samples are all submitted at the correct concentration. The number of reads for this service are guaranteed across all submitted samples, not per sample. We endeavour to provide at least 1000 reads per sample, however if a sample is at a significantly lower concentration than required we will not be able to guarantee read numbers for that sample.

How does library sequencing work?

During the library preparation of your library sequencing samples we attach barcodes and adapters to the ends of your DNA fragments. This allows us to sequence very small fragments (down to 50 bp) and we are able to get full length linear reads of PCR products or other linearised DNA.