A Wide Variety of Nanopore Sequencing Services
We offer a set of low-cost, standard Nanopore sequencing services.
You can also contact us to discuss any custom sequencing projects that you have.
During the library preparation of your plasmid samples the plasmids are linearised at random positions around their circumference and these linear fragments are then sequenced. Amplicon sequencing uses the same library preparation method and cuts randomly along the linear fragments and these fragments are then sequenced. We then run these sequencing reads through our assembly pipelines.
Most importantly, we provide assembled sequences (.final.fasta) and annotated genbank files of your plasmids and amplicons! We also provide some QC metrics that you can use to troubleshoot any issues.
Compared to short Sanger reads, we sequence entire plasmids and amplicons and provide you with assembled sequences, annotated maps and quality scores. This helps you to identify any assembly errors or mutations in your construct and be confident in your downstream experiments.
Knowing what you’re working with will save you time and money.
Library Sequencing
Sequence any linear DNA library (clonal or non clonal) for only £25 per sample.
During the library preparation of your library sequencing samples we attach barcodes and adapters to the ends of your DNA fragments. This allows us to sequence very small fragments (down to 50 bp) and we are able to get full length linear reads of PCR products or other linearised DNA.
We provide you with raw sequencing reads (.fastq.gz) and we can also provide consensus sequences.
Our library sequencing is perfect for getting the consensus sequences of a pool of amplicons or for doing a quick quality check before requesting greater read depth.
Custom Sequencing
Whole Genome Sequencing? AAV Sequencing? Or something not listed here? Get in touch with us about any other sequencing projects at contact@fullcirclelabs.bio
We sequence genomes, AAVs, environmental samples and more! We will give (almost) anything a shot.
Yes, that is where we believe that we add the most value. We have substantial experience in the design and analysis of sequencing experiments.
We generally return results within 7 days. For urgent projects we also offer an expedited service, where we start sequencing on the day that the samples arrive.
